L.G. Chitarra : Fluorescence techniques to detect bacteria

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28 Mar 2001 13:30
Unit: Wageningen University
Co Promotor: dr.ir. R.W. van den Bulk (Plant Research International), dr. T. Abee

 The conventional methods to detect and to assess the viability of plant pathogenic bacteria are usually based on plating assays or on serological techniques. Plating assays provide information about the number of viable cells, however, are time-consuming and laborious. Serological methods such as enzyme-linked immunosorbent assay (ELISA) and immunofluorescence microscopy (IF) can be performed in a shorter timespan, but they do not discriminate between live and dead cells and are not sensitive enough. Flow cytometry (FCM) in combination with fluorescent probes technology is a rapid, sensitive, quantitative and therefore, a promising technique to detect and to assess the viability of micro-organisms. FCM has been applied successfully to detect and distinguish between viable and non-viable bacteria in the fields of food microbiology, veterinary science and medical research. Luiz. G. Chitarra focuses on the development of a rapid, reliable, and accurate detection and assessment FCM technique in combination with fluorescent probes.
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