Mw. L.M. Verdelho Trindade : Potato life-cycle

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21 Feb 2003 16:00
Unit: Wageningen University
Location: Aula (gebouw 362), Gen. Foulkesweg 1, Wageningen
Website: http://www.gcw.nl/dissertations/
Promotor: prof.dr. R.G.F. Visser (Plant Breeding (Genetical Variation and Reproduction))prof.dr.ir. E. Jacobsen (Plant Breeding)

 During their life-cycle, plants need to integrate a wide range of tissue, developmental and environmental signals to regulate complex patterns of gene expression. A major level at which gene expression is regulated is the initiation of transcription, where promoters play an important role. The promoters are localised in the 5' end of each gene and they are arranged in several motifs or cis-acting elements where proteins bind in order to control gene expression. In potato, there is a special interest in promoters that confer specific expression in tuber related tissues. As not many potato promoters have been isolated and characterised it is difficult to predict which cis-acting elements are responsible for high and specific expression in tubers. Therefore a set of 16 specific promoters was isolated and 4 of these were functionally characterised by fusion with a reporter gene. In order to identify specific cis-acting elements a computer program called PRECISE (Prediction of Regulatory CIS-acting Elements) was developed. This computer program was tested on Arabidopsis promoters and the results showed its great power in the prediction of both know and putative cis-acting elements.
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