Mw.dr. I.M. Rienties : Proteins interacting with the Arabidopsis thaliana

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2 Jun 2003 16:00
Unit: Wageningen University
Location: Aula (gebouw 362), Gen. Foulkesweg 1, Wageningen
Promotor: prof.dr. S.C. de Vries (Biochemistry (Molecular Enzymology))

 The Somatic Embryogenesis Receptor-like Kinase 1 (AtSERK1) is a receptor-like kinase (RLK) that is proposed to participate in a signal transduction cascade involved in ovule and embryo development. The downstream components of the AtSERK1 signal transduction cascade are mostly unknown. Using the yeast two-hybrid system we found proteins that interact with the kinase domain of AtSERK1 and that can be used by AtSERK1 as substrate. These proteins were predicted to encode the 14-3-3 protein GF14?, the ATPase AtCDC48 and a putative RNA binding protein (RBP). The interactions were confirmed in vitro and in vivo. They showed that GF14? and AtDC48 preferably bind to the phosphorylated AtSERK1 kinase domain. In yeast, AtCDC48 is also able to interact with the AtSERK1-binding partners GF14? and the protein phosphatase KAPP. The interaction between AtCDC48 and a 14-3-3 protein was confirmed in plant cells. Based on the function and the cellular localisation of AtCDC48 we propose that AtSERK1 is involved in one of the many controlling pathways of the cell cycle.
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