The Campo-Ma’an rain forest in Cameroon has a high conservation value at local, national, regional and global levels. Despite its rich and diverse flora, the site is seriously affected by human activities that have led to deforestation and habitat fragmentation. A botanical inventory was carried out in the area to provide baseline biological information, essential for the elaboration of the Campo-Ma’an National Park management plan. During this study, a vegetation map of the Campo-Ma’an area was produced and the various vegetation types were described. A plant species checklist made of 2297 species, 851 genera and 155 families of vascular plants, as well as a list of 92 threatened species and 141 plant species of high conservation priorities such as endemic, rare, and species new to science were also produced. Biodiversity hotspots were identified and recommendations were given for its conservation and the sustainable management of its natural resources.