Dhr. Bjarne Rask Poulsen : Characterization of pathway engineered strains of filamentous fungi

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1 Apr 2005 13:30
Unit: Wageningen University
Location: Aula (gebouw 362), Gen. Foulkesweg 1, Wageningen
Promotor: prof.dr.ir. J. Tramper (Bioprocess Engineering)
Co Promotor: M.Vetr.Sc., PhD J.J.L. Ivesen (University of Southern Denmark), Dr. G.J.G. Ruijter (Leids Universitair Medisch Centrum), Dr.ir. J. Visser (Universiteit Leiden)

 Moulds are used by the industry for making a variety of products, e.g. penicillin, high-protein food, washing powder and soft drinks. For synthesis of many of these commodities the moulds require compounds, so-called reducing equivalents, to convert substrates to products. The synthesis is often slow because of too little reducing equivalents. We aimed to improve a mould by making it produce more of the enzymes that catalyse formation of reducing equivalents. To measure the effect(s) of more enzyme we compared the improved moulds with the unchanged mould by growing them in well-defined cultures. These were obtained in a tank with an extra mixer for flushing down the mould from the wall, where it otherwise settles in an inactive layer. Furthermore techniques were developed for automatic and frequent sampling of the mould, growth analysis, morphology profiling and continuous culturing. We succeeded to increase the level of reducing equivalents in the mould up to a factor of 9.
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