Ms S. Moço: "Metabolomics Technologies applied to the Identification of Compounds in Plants"

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15 Oct 2007 16:00
Unit: Wageningen University
Location: Aula, gebouw 362, Gen. Foulkesweg 1, Wageningen
Organisation: Wageningen University
Promotor: prof.dr. R.J. Bino (Metabolomica of Plants)
Co Promotor: Prof.dr. S.C. de Vries (Promotor), Dr. J.J. Vervoort, Dr. R.C.H. de Vos

The nutritional value and taste of plants and fruits are related to their chemical composition. The presence of “small molecules” (or metabolites) as plant constituents, influence their appearance and quality. In this thesis, approaches were developed for detecting and identifying metabolites in plants, in particular in tomato fruit. Technologies such as liquid chromatography coupled to mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) allowed a large scale characterization of metabolites from plant extracts. Databases of metabolites, based on LC-MS and NMR data, were obtained. A tomato fruit metabolite database (MoTo DB) was created which can be used as base for the development of dedicated databases. In fact, the techniques and methods established in this thesis can be extrapolated for the study of other crops.
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