N. (Nicolas) Champouret: Use of pathogenic genes from potato late blight to identify and characterise novel resistance genes in wild potato species.

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9 Jun 2010 16:00
Unit: Wageningen University
Location: Aula, building 362, Gen. Foulkesweg 1, Wageningen
Organisation: Wageningen University
Promotor: prof.dr. R.G.F. Visser (Genetical Variation and Reproduction)
Promotor: Prof.dr.ir. E. Jacobsen
Co Promotor: Dr.ir. V.G.A.A. Vleeshouwers

 Ever since the introduction of the late blight pathogen in potato growing areas, it forms a major threat for global potato production, despite extensive use of fungicides. Up-to-date strategies for late blight disease management require deployment of multiple resistance genes. These can be retrieved from wild species related to potato, but identification and characterisation is a long-lasting process. Therefore, more efficient gene isolation pipelines are required to identify functionally relevant resistance genes. In this thesis we use pathogenic genes from late blight to retrieve natural resistance genes in a broad range of potato relatives. This resulted in: (i) the development of genetic tools useful for late blight population monitoring in potato farming area using the broad spectrum resistance gene Rpi-blb1, (ii) the identification and characterisation of new variants of two resistances gene families in Mexican species, with potentially more valuable resistance gene for potato breeders, since some of them confer resistance to late blight with some specificity, (iii) new insights in the evolution of resistance genes families in its natural environment, and (iiii) the validation of a new approach (effectorormics) to accelerate resistance gene identification and characterisation.
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